[Histonet] Ventana antibody - H pylori

[Susan Foreman]

We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra
using UltraView DAB detection.  He have had dirty staining for spirochetes
(syphilis) in the past utilizing the Red Detection kit and decons do seem to
help a bit.  The DAB detection kit is much cleaner.  

-----Original Message-----
From: Sebree Linda A [mailto:LSebree at uwhealth.org] 
Sent: Thursday, July 09, 2015 5:16 PM
To: 'Cartun, Richard'; Histology Technician; Histonet;
histonet-request at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Rabbit monoclonal, Richard.

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm
A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-----Original Message-----
From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org]
Sent: Thursday, July 09, 2015 4:06 PM
To: Histology Technician; Histonet;
histonet-request at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic
Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Histology Technician [mailto:histology81176 at att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-request at lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

 Does anyone else have constant problems with the Ventana antibody H pylori
having a dirty background stain?  My pathologists are constantly complaining
about it and when I call CS I get the same response...decon the instrument,
try a new lot number, etc... it's never truly resolved.  Any thoughts?
Thanks!
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