[Histonet] XGal staining

[Galina Deyneko]

 Dear All, and Anna.I am confused about the recommendation of post fixation (stained slides?) in glutaraldehyde-foormaldehyde solution. According many protocols and my experience the fresh tissues  should be fixed in in glutaraldehyde -formalin solution that is mproved the tissue morphology and staining quality.The best counterstaining is Nuclear Fast Red from Vector.   Galina DeynekoNovartis, Cambridge, MA 617-871-7613 w