[Histonet] Specimen loss

Bob Richmond rsrichmond at gmail.com
Thu Jul 9 12:45:24 CDT 2015

Steve McClain writes: "In UPMC protocol They wrap all specimens, ink the
tissue with eosin (erythrosine) at the grossing bench and cut two levels at
40 microns."

Do NOT use eosin for this purpose. It's highly fluorescent, and will
interfere with any method (such as FISH) that relies on fluorescence.

Safranin O works well for this purpose - placing the specimens on those
blue pads after marking them. It's easily available - this is the red
component of the Gram stain done by any microbiology service.

Bob Richmond
Samurai Pathologist
Maryville TN

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