[Histonet] Amyloid by Congo Red

[Michael Ann Jones]

What protocol and reagents are you using for the stain?
Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com






On 1/23/15, 10:43 AM, "Jeffrey Robinson"
<JRobinson <@t> pathology-associates.com> wrote:

>Greetings to all Histotechs-  Here's an amyloid question for the
>braintrust.  We are cutting our slides and controls at 9 and staining in
>Congo Red for 1 hour.  The control stains fine but the patient tissue is
>staining negative even on cases that the pathologist assures us should be
>positive for amyloid.  We are using the Leica APEX charged slides with
>control and patient tissue on the same slide.  Does anyone have any
>thoughts on why the patient tissue is not staining?  Thanks!
>
>Jeff Robinson HT, HTL, Senior Histotechnologist, Sierra Pathology Lab,
>Clovis, CA.
>
>
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