[Histonet] Thermo IHC

[Cheri Miller]

Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can anyone help us cut through the *****88*888 and give me some helpful tips?  We have practical experience on the Ventana systems only. Has anyone been successful at using only 1 HIER buffer? Any tips on how to shorten the offline retrieval process? Currently we are at over 40 mins. Seems to me a ph of 7.6 -7.8 should work for most all of our antibodies. Thanks, Cheri

Cheryl A. Miller HT ASCP cm
Physicians Laboratory, P.C.
4840 F St.
Omaha , NE. 68117
402 731 4145 ext. 532
Cell 402 980 2537
Fax 402 731 8653

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