[Histonet] Special Stainer
Blake Taylor
bcdukes <@t> lexhealth.org
Tue Jan 20 12:27:57 CST 2015
We previously had the Ventana Nexus which was excellent, preferred it greatly over Dako but now we moved to the Ventana Benchmark Special Stainer and it has been nothing but trouble. I honestly at this point would not recommend the Benchmark. It needs improvement.
Blake Taylor
Surgical Pathology Supervisor
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Tuesday, January 20, 2015 1:01 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 134, Issue 23
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Today's Topics:
1. RE: Recycling alcohol (Marcum, Pamela A)
2. Decon HydeAway (Vickroy, James)
3. RE: specials stainers (Jeffrey Robinson)
4. Re: Recycling alcohol (Esther C Peters)
5. FW: Iron control tissue or blocks (Montana, Maria)
6. Re: Re: Recycling alcohol (Pam Marcum)
7. RE: specials stainers (Marcum, Pamela A)
----------------------------------------------------------------------
Message: 1
Date: Tue, 20 Jan 2015 14:22:17 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: [Histonet] RE: Recycling alcohol
To: "Horn, Hazel V" <HornHV <@t> archildrens.org>, "'Vickroy, James'"
<jvickroy <@t> SpringfieldClinic.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4c96ad5c746b4ba5ba60615893a16b48 <@t> MAIL13M2N2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage.
Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, January 19, 2015 1:05 PM
To: 'Vickroy, James'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol
With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Monday, January 19, 2015 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Recycling alcohol
We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%.
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvickroy <@t> SpringfieldClinic.com<mailto:jvickroy <@t> SpringfieldClinic.com>
This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.
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Message: 2
Date: Tue, 20 Jan 2015 15:10:08 +0000
From: "Vickroy, James" <jvickroy <@t> SpringfieldClinic.com>
Subject: [Histonet] Decon HydeAway
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<9B1A1501A800064397369BD8072E6BCA96C365 <@t> E2K10DB.springfieldclinic.com>
Content-Type: text/plain; charset="us-ascii"
Anybody using Decon HydeAway for neutralization of 10% NBF. If so what are your experiences and do you dispose of the product (gel) into your solid biological waste for disposal?
In the past I have used Formalex or Formalex Green. This material looks cheaper but cheaper is not always better of course.
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvickroy <@t> SpringfieldClinic.com<mailto:jvickroy <@t> SpringfieldClinic.com>
This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.
------------------------------
Message: 3
Date: Tue, 20 Jan 2015 16:31:24 +0000
From: Jeffrey Robinson <JRobinson <@t> pathology-associates.com>
Subject: [Histonet] RE: specials stainers
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<204A03EB5A7F0A4BB1EEDD52A963829C16D8AF38 <@t> PAEXCH1.PathologyAssociates.local>
Content-Type: text/plain; charset="us-ascii"
I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle.
Jeff Robinson, HT, HTL
Sierra Pathology Lab, Clovis, CA
jrobinson <@t> pathology-associates.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, January 20, 2015 6:13 AM
To: 'Mitchell, Janice A'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: specials stainers
Oops - I had IHC on the brain and didn't pay close attention!
Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho.
I very much recommend the DAKO Artisan..
It is Monday on Tuesday... right?
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
-----Original Message-----
From: Weems, Joyce K.
Sent: Tuesday, January 20, 2015 8:59 AM
To: 'Mitchell, Janice A'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: specials stainers
I recommend Leica.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] specials stainers
Good Morning,
We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts?
Thanks for any input, Janice Mitchell
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Message: 4
Date: Tue, 20 Jan 2015 16:35:39 +0000
From: Esther C Peters <epeters2 <@t> gmu.edu>
Subject: [Histonet] Re: Recycling alcohol
To: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>, "Horn, Hazel V"
<HornHV <@t> archildrens.org>, "'Vickroy, James'"
<jvickroy <@t> SpringfieldClinic.com>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1421771738729.88183 <@t> gmu.edu>
Content-Type: text/plain; charset="iso-8859-1"
American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol.
Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-4444
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epeters2 <@t> gmu.edu
https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Marcum, Pamela A <PAMarcum <@t> uams.edu>
Sent: Tuesday, January 20, 2015 9:22 AM
To: Horn, Hazel V; 'Vickroy, James'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol
Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage.
Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, January 19, 2015 1:05 PM
To: 'Vickroy, James'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol
With Ethyl alcohol you will need a license and will have to keep records. With reagent grade alcohol none of that is necessary.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Monday, January 19, 2015 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Recycling alcohol
We are planning to recycle alcohol in the new lab I am working with. Previously I always used an alcohol blend such as the "Flex" products. However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. We will be getting our alcohol from Thermofisher. Can anyone tell me which alcohols they are using for dehydration? Reagent alcohol or ethyl alcohol. Obviously we will make our own concentrations from a 100%.
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois 62703
Office: 217-528-7541, Ext. 15121
Email: jvickroy <@t> SpringfieldClinic.com<mailto:jvickroy <@t> SpringfieldClinic.com>
This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Message: 5
Date: Tue, 20 Jan 2015 10:36:02 -0600
From: "Montana, Maria" <mtmontana <@t> primecare.org>
Subject: [Histonet] FW: Iron control tissue or blocks
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1E0E2B14C709174B8AC2BE0AE7F76833A464DB0062 <@t> EXCHANGE2K7.staprimecare.org>
Content-Type: text/plain; charset="iso-8859-1"
________________________________
From: Montana, Maria
Sent: Wednesday, January 07, 2015 1:50 PM
To: hisonet <@t> lists.utsouthwestern.edu
Subject: Iron control tissue or blocks
Hi, Histoneters,
I was wondering if anyone out there has some good Fe control tissue or blocks that you would be willing to trade for. We have Amyloid tissue, if you are interested.
Thanks,
Maria Montana HTL (ASCP)
CHI St Alexius Health
(701)530-6732
________________________________
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Message: 6
Date: Tue, 20 Jan 2015 16:39:40 +0000 (UTC)
From: Pam Marcum <mucram11 <@t> comcast.net>
Subject: Re: [Histonet] Re: Recycling alcohol
To: Esther C Peters <epeters2 <@t> gmu.edu>
Cc: Hazel V Horn <HornHV <@t> archildrens.org>, James' 'Vickroy
<jvickroy <@t> SpringfieldClinic.com>, "Marcum, Pamela"
<PAMarcum <@t> uams.edu>, Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<295469677.1387992.1421771980759.JavaMail.zimbra <@t> comcast.net>
Content-Type: text/plain; charset=utf-8
The issue is of isoprpanol and/or methanol is really not the point.?? The ATF requires the alcohol to be unfit for human consumption so either or both of these solutions in ethanol will render it dangerous and undrinkable.?? If you do not have one or both of these in the ethanol it is taxed and therefore very expensive and time consuming.
??
Pam
----- Original Message -----
From: "Esther C Peters" <epeters2 <@t> gmu.edu>
To: "Pamela A Marcum" <PAMarcum <@t> uams.edu>, "Hazel V Horn" <HornHV <@t> archildrens.org>, "James' 'Vickroy" <jvickroy <@t> SpringfieldClinic.com>, "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, January 20, 2015 10:35:39 AM
Subject: [Histonet] Re: Recycling alcohol
American Mastertech Scientific makes Reagent Alcohol with just ethanol and isopropanol.
Esther C. Peters, Ph.D.
Term Associate Professor
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-4444
Office: David King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066
e-mail: epeters2 <@t> gmu.edu
https://bluprd0511.outlook.com/owa/redir.aspx?C=ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKrrDjhwOvk.&URL=http%3a%2f%2fesp.gmu.edu
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu <histonet-bounces <@t> lists.utsouthwestern.edu> on behalf of Marcum, Pamela A <PAMarcum <@t> uams.edu>
Sent: Tuesday, January 20, 2015 9:22 AM
To: Horn, Hazel V; 'Vickroy, James'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol
Hazel is correct if you use the pure ethanol the paperwork is horrible and the limits on what should be in the lab are very low for a normal Histology Lab. ??Reagent alcohol is the best way to go as it has methanol and isopropanol in it so it is undrinkable and therefore not under the same ATF rules of usage.
Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Horn, Hazel V
Sent: Monday, January 19, 2015 1:05 PM
To: 'Vickroy, James'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Recycling alcohol
With Ethyl alcohol you will need a license and will have to keep records. ??With reagent grade alcohol none of that is necessary.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv <@t> archildrens.org
archildrens.org
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Vickroy, James
Sent: Monday, January 19, 2015 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Recycling alcohol
We are planning to recycle alcohol in the new lab I am working with. ?? Previously I always used an alcohol blend such as the "Flex" products. ??However at this new lab we are going to only process biopsies so I believe I can get by using ethanol and not a blend. ?? We will be getting our alcohol from Thermofisher. ??Can anyone tell me which alcohols they are using for dehydration? ??Reagent alcohol or ethyl alcohol. ??Obviously we will make our own concentrations from a 100%.
Jim
Jim Vickroy
Histology Manager
Springfield Clinic, Main Campus, East Building
1025 South 6th Street
Springfield, Illinois ??62703
Office: ??217-528-7541, Ext. 15121
Email: ??jvickroy <@t> SpringfieldClinic.com<mailto:jvickroy <@t> SpringfieldClinic.com>
This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you.
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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Thank you.
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------------------------------
Message: 7
Date: Tue, 20 Jan 2015 16:51:22 +0000
From: "Marcum, Pamela A" <PAMarcum <@t> uams.edu>
Subject: [Histonet] RE: specials stainers
To: "'Jeffrey Robinson'" <JRobinson <@t> pathology-associates.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <09c58db6864242c2a9f4371873e68265 <@t> MAIL13M2N2.ad.uams.edu>
Content-Type: text/plain; charset="us-ascii"
We use our Leica H&E stainer for PAS only as it is the fastest way we have found to get 30 to 40 bone marrow PAS slides out before 6:30AM every morning. Otherwise it was just too cumbersome and the Dako does a great job on all routine stains. We only do the neuro stains and Copper by hand now. The Dako Copper kit is a different procedure and our pathologist like so it can't be used. The PAS is just too slow for the turnaround we need daily. The stain is great!!
Pam Marcum
UAMS
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Tuesday, January 20, 2015 10:31 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: specials stainers
I have the Leica multistainer. We use it for H & E staining only. When we attempted to set it up for Special Stains, all of the silver stains had to be made up either each run or each day. Too much hassle.
Jeff Robinson, HT, HTL
Sierra Pathology Lab, Clovis, CA
jrobinson <@t> pathology-associates.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, January 20, 2015 6:13 AM
To: 'Mitchell, Janice A'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: specials stainers
Oops - I had IHC on the brain and didn't pay close attention!
Leica does not have a dedicated special stainer - but they have a multistainer which stains specials as well as H&E. Is too slow in a busy lab tho.
I very much recommend the DAKO Artisan..
It is Monday on Tuesday... right?
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph's Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
-----Original Message-----
From: Weems, Joyce K.
Sent: Tuesday, January 20, 2015 8:59 AM
To: 'Mitchell, Janice A'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: specials stainers
I recommend Leica.
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
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From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mitchell, Janice A
Sent: Tuesday, January 20, 2015 6:43 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] specials stainers
Good Morning,
We are looking for an automatic stainer for special stains. Ventana vs Dako? Thoughts?
Thanks for any input, Janice Mitchell
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End of Histonet Digest, Vol 134, Issue 23
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