RE: [Histonet] preferable thickness of sections for diagnostic demonstration of neurodegenerative pathologies

[naje1972]

6,8,10or 20 micron sections should work for you 
Hope this helps
Cynthia James



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-------- Original message --------
From: Tyrone Genade <tgenade <@t> gmail.com> 
Date: 01/15/2015  5:56 PM  (GMT-06:00) 
To: histonet <histonet <@t> lists.utsouthwestern.edu> 
Subject: [Histonet] preferable thickness of sections for diagnostic
  demonstration of neurodegenerative pathologies 
 
Hello,

I am planning a study and need advice on the optimum tissue thickness for
diagnosis of:
alpha-synucleinopathies
tauopathies
amyloid plaques

The study is primarily comparative distribution and frequency between age
groups and would be looking to compare "affected neurons per field of
vision/total neurons visible" rather than stereological cell counts (which
are beyond my budget at this time).

I plan on using fixed paraffin embedded tissue. The GE site (
http://www.clarientinc.com/test-menu.aspx?AlphabeticalFilter=A&TestID=2571
) suggest 4 micron for Lewy Bodies. Any particular reason?

Thanks
-- 
Tyrone Genade
Orange City, Iowa
tel: (+1) 712 230 4101
http://tgenade.freeshell.org
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