[Histonet] marking small samples

[Melissa Burns]

I've been told Eosin affects a lot of molecular testing. We used to use it in our process but discontinued to not interfere with future testing.  

Sent from my iPhone

> On Jan 14, 2015, at 5:31 PM, "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu> wrote:
> 
> We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx
> 
> Tim Morken
> Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
> UC San Francisco Medical Center
> San Francisco, CA
> 
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> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yves Heremans
> Sent: Wednesday, January 14, 2015 2:03 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] marking small samples
> 
> Dear Histonetters,
> 
> Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ?
> Preferably something that would not interfere with subsequent antibody staining.
> 
> Yves
> 
> 
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