[Histonet] marking small samples

Morken, Timothy Timothy.Morken <@t> ucsf.edu
Wed Jan 14 16:30:12 CST 2015


We use eosin the processor alcohol. Also we use blue-tinted paraffin which helps with contrast on small bx

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Yves Heremans
Sent: Wednesday, January 14, 2015 2:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking small samples

Dear Histonetters,

Does anyone know of a good method to mark (stain) small samples (tissue or cells) prior to paraffin embedding to aid in finding back more easily the sample in the paraffin block ?
Preferably something that would not interfere with subsequent antibody staining.

Yves


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