[Histonet] Bone Tissue in plastic embedding

Fri Jan 9 14:03:10 CST 2015

Dear Histonetter's,

Happy new year to everyone! I come today with a couple of questions regarding human bone embedded  in MMA:

1.       What is a good technique to dissolve the MMA from the tissue section? Also any suggestions on how to stain with H&E?

2.       After mounting my tissue sections (5-10microns) with Haupts adhesive, I noticed that the bone within the section was appearing opaque/darkened on the microscope? Any suggestions on what this might be?

Thank you very much,

-Jesse H.