[Histonet] RE: PASD muscle stains

[Cooper, Brian]

Same here. 

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
bcooper <@t> chla.usc.edu 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Linda Prasad (SCHN)
Sent: Tuesday, January 06, 2015 2:58 PM
To: 'Tiffany Passaro'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] RE: PASD muscle stains

Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 minutes. Don't use any fixatives :)


Linda Prasad, MSc, BSc

Senior Scientist | Histopathology
t: 02 9845 3306 | f: 02 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au
m: 0425 314 267
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro
Sent: Tuesday, 6 January 2015 10:23 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] PASD muscle stains

Greetings,


                I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this.

Tiffany
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