[Histonet] RE: PASD muscle stains

[Linda Prasad (SCHN)]

Usually we do the DiPAS stain on muscle frozen section. Air dried for 10 minutes. Don't use any fixatives :)


Linda Prasad, MSc, BSc

Senior Scientist | Histopathology
t: 02 9845 3306 | f: 02 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au
m: 0425 314 267
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tiffany Passaro
Sent: Tuesday, 6 January 2015 10:23 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] PASD muscle stains

Greetings,


                I am looking for fixatives that others are using in their labs for the PASD stain on fresh frozen muscle tissue. Currently we are fixing in 10% NBF. Thanks in advance for any info on this.

Tiffany
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