[Histonet] Cryostat troublshooting. CM3050

[Lewis, Patrick]

H everyone,

We have been using this old CM3050 cryostat for our Frozen OCT blocks.

I am cutting at 5 uM.

My issue is that when I use the hand rotator to cut my sections it doesn't seem to advance with each rotation.  It can take as many as 4 rotations to go from one section to another.

What would cause this, and is there an easy solution to this problem?

I can use the button to automatically move the chick holder all the forward and back from blinking to blinking, so I am wondering if ice crystals or debris can be ruled out as  the issue.

Any advice welcome.

Thanks

Patrick.

ps: when I do get the blade to cut into the block, I have to wonder if the section I am getting is really 5 uM, or is it thicker.  Sometimes you can tell if the section looks particularly thick, but other times it's hard to tell.


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