[EXTERNAL] RE: [Histonet] cutting honey bees

[Roy, Ryan]

I agree with Patsy. If you have a access to a "plastic histo lab",  that would be the best, as you could easily section any part of the Bee's anatomy. 

Ryan Roy HTL (ASCP)
Manchester Veterans Affairs Medical Center
Manchester New Hampshire

Disclosure: The content of this email does not represent the views or opinons of the VA



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, January 06, 2015 2:13 PM
To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
Subject: [EXTERNAL] RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com



> From: rjr6 <@t> psu.edu
> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Date: Sat, 3 Jan 2015 23:15:33 +0000
> Subject: RE: [Histonet] cutting honey bees
> CC: 
> 
> I sectioned and stained honey bee and yellow jacket stingers years ago.  They wanted to show the difference between the stingers.  I wasn't sure what to do so I processed and handled like everything else.  I was able to get some good sections.  I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg 
> [classicdoc <@t> gmail.com]
> Sent: Saturday, January 03, 2015 6:08 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] cutting honey bees
> 
> Has anyone had experience embedding and cutting honey bees. I am sure 
> there are some issues with the harder exoskeleton. Would that have to 
> be dissected away first. I am considering helping a student with a 
> science fair project on bees.
> 
> Douglas Gregg
> Veterianary pathologist
> 
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