[Histonet] cutting honey bees

[Grantham, Andrea L - (algranth)]

I processed honeybees and was successful sectioning them. It takes a bit of patience and time. I soaked the bees to soften the outer parts in glycerin water and or mollifex. As for processing, I had to make up a schedule and infiltration is the key. If it isn't important to see the whole bee you can section out the parts you need to see most and then it becomes easy.

I'm slowly working on my notes and someday I'll get them all sorted out.

Andi G
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Patsy Ruegg [pruegghm <@t> hotmail.com]
Sent: Tuesday, January 06, 2015 12:13 PM
To: Roberta Horner; Douglas Gregg; Histonet <@t> Lists. Edu
Subject: RE: [Histonet] cutting honey bees

for the whole bee I probably would process and embed it in glycol methacrylate (gma) it is much harder and would give better sections, we have done zebra fish and several other harder tissues including calcified bone in GMA.

Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com



> From: rjr6 <@t> psu.edu
> To: classicdoc <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Date: Sat, 3 Jan 2015 23:15:33 +0000
> Subject: RE: [Histonet] cutting honey bees
> CC:
>
> I sectioned and stained honey bee and yellow jacket stingers years ago.  They wanted to show the difference between the stingers.  I wasn't sure what to do so I processed and handled like everything else.  I was able to get some good sections.  I put 6 stingers in each block and cut several sections figuring there should be at least one good stinger in each block and it worked.
> Roberta Horner
> Penn State University
> Animal Diagnostic Lab
> ________________________________________
> From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Douglas Gregg [classicdoc <@t> gmail.com]
> Sent: Saturday, January 03, 2015 6:08 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] cutting honey bees
>
> Has anyone had experience embedding and cutting honey bees. I am sure
> there are some issues with the harder exoskeleton. Would that have to
> be dissected away first. I am considering helping a student with a
> science fair project on bees.
>
> Douglas Gregg
> Veterianary pathologist
>
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