[Histonet] RE: cilia for EM

[Morken, Timothy]

Our clinicians use a brush of some sort. Unfortunately I don't know the brand. But the bristles do not come off; they are pretty stout, are in a spiral pattern and each bristle has a knob at the end. We just gently scrape the cells off the brush into fixative in a petri dish and then pipet up and put in a several microtubes for centrifugation.

Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A
Sent: Thursday, February 26, 2015 9:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] cilia for EM

Does anyone have a good technique/tool for cilia biopsy collection that we could share with our physicians.  We have been having issues with poor quality specimens.
Thanks,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo <@t> providence.org<mailto:lacie.algeo <@t> providence.org>


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