[Histonet] gelatin

[John Kiernan]

Dear ynwang <@t> u.washington.edu.

Boiling makes steam, with bubbles that greatly change tissue structure. Slow freezing is just as bad; the ice crystals make holes that deform and replace the tissue architecture. What are you tryng to find out? It has been known for 100+ years that boiling collagen makes gelatin, and further concentration makes traditional glue. 
 You should involve your boss in future email exchanges.
John Kiernan
London, Canada
= = =
On 24/02/15, Yak-Nam Wang  <ynwang <@t> u.washington.edu> wrote:
> Thank you for your e-mail.
> 
> 
> Apologies for not explaining "treated tissue". We treat the tissue with high intensity focused ultrasound. It can raise the temperature of tissue to boiling in a localized area (millimeter areas). I could use a biochemical assay for collagen and gelatin if we treat a large area, but with single lesions I was hoping I could visualize this. In some treated areas we are almost resulting in liquefaction of the tissue. I am interested to see if we are turning the collagen to gelatin in these areas and what part of the lesion this is happening. You should involve your boss in future email exchanges.
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> Thank you for your thoughts
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> Yak-Nam
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> On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan <jkiernan <@t> uwo.ca> wrote:
> 
> > You need to explain "treated tissue". 
> > 
> > Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope. 
> > 
> > If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges.
> > 
> > John Kiernan
> > London, Canada
> > = = =
> > On 23/02/15, Yak-Nam Wang  <ynwang <@t> u.washington.edu> wrote:
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> > > Hello,
> > > 
> > > Does anyone know of a stain specific for gelatin? I would like to
> > > distinguish between firbous collagen and gelatin in treated tissue.
> > > 
> > > thank you
> > > 
> > > Yak-Nam
> > > 
> > > University of Washington
> > > Seattle, WA
> > > 
> > > 
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