[Histonet] Re: gelatin
Galina Deyneko
galinadeyneko <@t> yahoo.com
Tue Feb 24 13:21:24 CST 2015
Thank you Dr. Kiernan, Nice and scientific explanation how to prepare gelatin. I beleive that a lot of house wives and cooks know how to prepare meet or fish jelly (long bouling of the bones with cartilage and tendons). very popular in russian or easter europian cuisine, called "cholodez" which neans "cold".best regards Galina DeynekoNovartis, Cambridge, MA 617-871-7613 w
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Subject: Histonet Digest, Vol 135, Issue 25
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Today's Topics:
1. Re: Storing antibodies in a frostless freezer (Teri Johnson)
2. Re: gelatin (John Kiernan)
3. RE: [EXTERNAL] Re: [Histonet] gelatin (Roy, Ryan)
4. How dark is dark enough? (Paula Sicurello)
5. Re: gelatin (Yak-Nam Wang)
6. Re: How dark is dark enough? (Rene J Buesa)
7. Creutzfeldt-Jakob Disease (Scott, Allison D)
8. RE: Creutzfeldt-Jakob Disease (Debra Siena)
9. RE: How dark is dark enough? (Morken, Timothy)
10. CBG recycler (Roy, Ryan)
----------------------------------------------------------------------
Message: 1
Date: Mon, 23 Feb 2015 18:28:54 +0000
From: Teri Johnson <tejohnson <@t> genoptix.com>
Subject: [Histonet] Re: Storing antibodies in a frostless freezer
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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I agree with Rachel. I would not be quite as worried about the temperature change if indeed they maintain no higher than -13 degrees C and would not be freeze/thawing, but what does your ice cube tray look like after about a month or more in a frost-free freezer?
Best wishes,
Teri Johnson, HT(ASCP)QIHC
Genoptix, Inc.
A Novartis Company
Carlsbad, CA
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Message: 2
Date: Tue, 24 Feb 2015 00:47:50 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] gelatin
To: Yak-Nam Wang <ynwang <@t> u.washington.edu>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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You need to explain "treated tissue".
Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope.
If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges.
John Kiernan
London, Canada
= = =
On 23/02/15, Yak-Nam Wang <ynwang <@t> u.washington.edu> wrote:
> Hello,
>
> Does anyone know of a stain specific for gelatin? I would like to
> distinguish between firbous collagen and gelatin in treated tissue.
>
> thank you
>
> Yak-Nam
>
> University of Washington
> Seattle, WA
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 3
Date: Tue, 24 Feb 2015 10:06:11 -0500
From: "Roy, Ryan" <Ryan.Roy <@t> va.gov>
Subject: RE: [EXTERNAL] Re: [Histonet] gelatin
To: 'John Kiernan' <jkiernan <@t> uwo.ca>, Yak-Nam Wang
<ynwang <@t> u.washington.edu>, "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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That's interesting, I didn't realize gelatin is the water soluable protein of collagen. No experience with staining gelatin, but have you considered MassonTrichrome.
It is used to differentiate collagen from smooth muscle...
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Kiernan
Sent: Tuesday, February 24, 2015 12:48 AM
To: Yak-Nam Wang; histonet <@t> lists.utsouthwestern.edu
Subject: [EXTERNAL] Re: [Histonet] gelatin
You need to explain "treated tissue".
Gelatin is collagen that has been boiled until the protein has lost all its fibrous nature and changed into a water-soluble protein. Gelatin is made permanently insoluble by adequate formaldehyde fixation. It is stained by anionic dyes (such as eosin in the H&E method), but it does not show as fibres when you look at the section or smear through a microscope.
If this doesn't answer your question, please explain your problem and involve your boss in future email exchanges.
John Kiernan
London, Canada
= = =
On 23/02/15, Yak-Nam Wang <ynwang <@t> u.washington.edu> wrote:
> Hello,
>
> Does anyone know of a stain specific for gelatin? I would like to
> distinguish between firbous collagen and gelatin in treated tissue.
>
> thank you
>
> Yak-Nam
>
> University of Washington
> Seattle, WA
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
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Message: 4
Date: Tue, 24 Feb 2015 07:21:12 -0800
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: [Histonet] How dark is dark enough?
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
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Good Morning Netters,
While running immunofluorescence stains, how dark is dark enough?
I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.
I feel that dimmed lights are good enough.
What does the histology collective think?
Thanks in advance!
Paula :-)
------------------------------
Message: 5
Date: Tue, 24 Feb 2015 07:30:19 -0800
From: Yak-Nam Wang <ynwang <@t> u.washington.edu>
Subject: Re: [Histonet] gelatin
To: John Kiernan <jkiernan <@t> uwo.ca>
Cc: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<CAOKiGw+65OpJ_z9dto84GOEPrZ4QTVRfRtPTXzmN56otmBEtxw <@t> mail.gmail.com>
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Thank you for your e-mail.
Apologies for not explaining "treated tissue". We treat the tissue with
high intensity focused ultrasound. It can raise the temperature of tissue
to boiling in a localized area (millimeter areas). I could use a
biochemical assay for collagen and gelatin if we treat a large area, but
with single lesions I was hoping I could visualize this. In some treated
areas we are almost resulting in liquefaction of the tissue. I am
interested to see if we are turning the collagen to gelatin in these areas
and what part of the lesion this is happening.
Thank you for your thoughts
Yak-Nam
On Mon, Feb 23, 2015 at 9:47 PM, John Kiernan <jkiernan <@t> uwo.ca> wrote:
> You need to explain "treated tissue".
>
> Gelatin is collagen that has been boiled until the protein has lost all
> its fibrous nature and changed into a water-soluble protein. Gelatin is
> made permanently insoluble by adequate formaldehyde fixation. It is stained
> by anionic dyes (such as eosin in the H&E method), but it does not show as
> fibres when you look at the section or smear through a microscope.
>
> If this doesn't answer your question, please explain your problem and
> involve your boss in future email exchanges.
>
> *John Kiernan*
> London, Canada
> = = =
>
> On 23/02/15, *Yak-Nam Wang * <ynwang <@t> u.washington.edu> wrote:
>
> Hello,
>
> Does anyone know of a stain specific for gelatin? I would like to
> distinguish between firbous collagen and gelatin in treated tissue.
>
> thank you
>
> Yak-Nam
>
> University of Washington
> Seattle, WA
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
------------------------------
Message: 6
Date: Tue, 24 Feb 2015 15:37:09 +0000 (UTC)
From: Rene J Buesa <rjbuesa <@t> yahoo.com>
Subject: Re: [Histonet] How dark is dark enough?
To: Paula Sicurello <patpxs <@t> gmail.com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<1238200618.5179661.1424792229766.JavaMail.yahoo <@t> mail.yahoo.com>
Content-Type: text/plain; charset=UTF-8
As long as your microscope has a good Hg source light, it is not really necessary to darken the room so much.�I used to have a very small room were the microscope was located but later on we moved the microscope to another area were we did not dim the light at all and the vision was OK.Everything depends on the circumstances and how comfortable�you are with the image.There are no rules�on this subject.Ren� J.�
On Tuesday, February 24, 2015 10:21 AM, Paula Sicurello <patpxs <@t> gmail.com> wrote:
Good Morning Netters,
While running immunofluorescence stains, how dark is dark enough?
I have worked in labs where we did them in full room light, almost complete
darkness (developing negative/film darkness), and somewhere in between.
I feel that dimmed lights are good enough.
What does the histology collective think?
Thanks in advance!
Paula� :-)
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------------------------------
Message: 7
Date: Tue, 24 Feb 2015 15:45:12 +0000
From: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>
Subject: [Histonet] Creutzfeldt-Jakob Disease
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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<FA607DC3D1ED7C46A9546820A3EB877F0A81BE21 <@t> LBMSG02.hchd.local>
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Hello to all in histoland. Does anyone have a procedure for handling creutzfeldt-jakob disease. Any help will be appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)
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Message: 8
Date: Tue, 24 Feb 2015 15:57:54 +0000
From: Debra Siena <DSiena <@t> statlab.com>
Subject: [Histonet] RE: Creutzfeldt-Jakob Disease
To: "Scott, Allison D" <Allison.Scott <@t> harrishealth.org>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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Hi Allison,
I would suggest going to the CDC website and pulling from there, they should have the latest recommendations. thanks
Debbie Siena
800.442.3573 ext. 229 | www.statlab.com
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Tuesday, February 24, 2015 9:45 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Creutzfeldt-Jakob Disease
Hello to all in histoland. Does anyone have a procedure for handling creutzfeldt-jakob disease. Any help will be appreciated.
Allison Scott HT(ASCP)
Histology Supervisor
LBJ Hospital
713-566-5287(Lab)
713-566-2148(Office)
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Message: 9
Date: Tue, 24 Feb 2015 16:06:37 +0000
From: "Morken, Timothy" <Timothy.Morken <@t> ucsf.edu>
Subject: RE: [Histonet] How dark is dark enough?
To: 'Paula Sicurello' <patpxs <@t> gmail.com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<761E2B5697F795489C8710BCC72141FF367F6B5C <@t> ex07.net.ucsf.edu>
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If you are using an automated stainer the plexiglas cover that they all have will block UV light. For instance we run all our IF in a Dako stainer with the slightly tinted Plexiglas and have not had any problems. If staining manually in a tray, covering with something to block light is ok. On the other hand, I can't really say I've had a problem even with no light blocking. The incubations are usually so short it may not make a difference. Consider that the focused UV light in the fluorescence scope is thousands of times stronger than any overhead tube and it still takes a while for the signal to dim.
Tim Morken
Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies
UC San Francisco Medical Center
San Francisco, CA
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Tuesday, February 24, 2015 7:21 AM
To: HistoNet
Subject: [Histonet] How dark is dark enough?
Good Morning Netters,
While running immunofluorescence stains, how dark is dark enough?
I have worked in labs where we did them in full room light, almost complete darkness (developing negative/film darkness), and somewhere in between.
I feel that dimmed lights are good enough.
What does the histology collective think?
Thanks in advance!
Paula :-)
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------------------------------
Message: 10
Date: Tue, 24 Feb 2015 11:41:41 -0500
From: "Roy, Ryan" <Ryan.Roy <@t> va.gov>
Subject: [Histonet] CBG recycler
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Cc: "Hall, Kevin P." <Kevin.Hall <@t> va.gov>
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<15F883394EAB744E99E1C7E1B98730490178A821D214 <@t> R04BYNMSGB1.r04.med.va.gov>
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Hello histonet,
Has anyone out there dealt with a busted Heat Resistor in a CBG reagent recycler.
Any thoughts or insights appreciated.
Thanks in advance,
Ryan Roy HTL (ASCP)
Manchester Veterans Affairs Medical Center
Manchester New Hampshire
Disclosure: The content of this email does not represent the views or opinons of the VA
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