[Histonet] RE: Help with cutting mouse brain at 9-10 Microns

[Marcum, Pamela A]

Please give your processing schedule with times and reagents?  It is hard to help without knowing how the tissue was handled from sacrifice to embedding.  Pam Marcum

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kimberly Marshall
Sent: Wednesday, February 18, 2015 8:20 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Help with cutting mouse brain at 9-10 Microns

?Hello Histo folks


  I am starting a research project with mouse brain,  I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all.  I have soaked them in warm water and wonder in using a  softener like conditioner would help.  I am new to the animal tissue world and any advise would help.


Thanks in advance.

Kimberly
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