[Histonet] Help with cutting mouse brain at 9-10 Microns

[Kimberly Marshall]

?Hello Histo folks


  I am starting a research project with mouse brain,  I am having trouble with chatter on the regular 3.5 mm sections and cant get the 9-10 mm to cut at all.  I have soaked them in warm water and wonder in using a  softener like conditioner would help.  I am new to the animal tissue world and any advise would help.


Thanks in advance.

Kimberly