[Histonet] RE: processing problem

[Jeffrey Robinson]

Hi Martha-  I have had some limited success in rehabilitating underprocessed tissue without reprocessing it in the tissue processor.  If the tissue samples are not too large you may be able to let them air dry for a period of time so that they dry out.  Make sure to face the blocks if you haven't already done so.  You can then melt them down and put them in a mold with liquid paraffin and just let them sit on the embeddeder (still in liquid paraffin) for a couple of hours for better infiltration.  Re-embed your samples and hope for the best.  I hope this helps.

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davenport, Martha R
Sent: Thursday, February 12, 2015 5:41 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] processing problem

I am Martha Davenport, supervisor, at the University of KY histology lab. We had a processing problem caused  by  accidently placing 70% (instead of 100%) into the last dehydration container. Would anyone please give me info on how you would remedy this?  We usually reprocess the tissue but have had trouble in the past with the tissue morphology being optimal. Any help will be greatly appreciated.
Martha Davenport 859-257-1822
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