[Histonet] processing problem

[Rene J Buesa]

Having morphology problems when "re-processing" a tissue after an accident as you had is the "unavoidable" consequence. The problem is rooted in the fact that usually you remove the paraffin using the tissue processor cleaning cycle and that is a very harsh method (albeit the most expeditious). If you  "re-process" using more steps than usual in the dehydration you may have very slightly better results, but not that much.The mistake you made have the consequences you fear.René J.  

     On Monday, February 16, 2015 12:08 PM, "Davenport, Martha R" <martha.davenport <@t> uky.edu> wrote:
   

 I am Martha Davenport, supervisor, at the University of KY histology lab. We had a processing problem caused  by  accidently placing 70% (instead of 100%) into the last dehydration container. Would anyone please give me info on how you would remedy this?  We usually reprocess the tissue but have had trouble in the past with the tissue morphology being optimal. Any help will be greatly appreciated.
Martha Davenport 859-257-1822
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