[Histonet] Question regarding dehydration

[Julio Benavides]

Hi there,

we are having problems trying to cut some embedded samples (they crumble 
in the bath and the few cuts we manage to get into HE are crap). These 
are formalin fixed samples (bovine foetal and placenta samples) which 
went straight from formlin into 100º ethanol for the dehydration before 
clearing. I was wondering if such drastic dehydration step (no 60º, 70º 
or 90º ethanol before the 100º) could have damage the tissue. Has anyone 
have a similar issue before? do you think the samples are ruined for 
histology?

Thanks a lot for your help

Regards

Julio
Instituto de Ganaderia de Montaña
Spain