[Histonet] Histonet Digest, Vol 145, Issue 24

[Cheryl]

Not bleach!  It'll change anything with melanin and other cellular component. 

Try formalin vapor (in a chamber in small batches).  Or if you must rinse them, 10% neutral buffered formalin or whatever your original primary fixative was prior to processing.  

Please excuse typos-sent from a phone. 

> On Dec 25, 2015, at 10:00 AM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
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>   1. fungus growing on paraffin blocks (Dube, Emily)
>   2. Vegf. (Laurie Finch)
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> ----------------------------------------------------------------------
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> Message: 1
> Date: Thu, 24 Dec 2015 19:32:56 +0000
> From: "Dube, Emily" <Emily.Dube at nyumc.org>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] fungus growing on paraffin blocks
> Message-ID: <0C41582E-A43E-4830-A08F-1D508411963F at nyumc.org>
> Content-Type: text/plain; charset="utf-8"
> 
> I work for a biobanking research study and recently we discovered some of our paraffin blocks had mold/fungus growing on the tissue. We were advised to soak the blocks in bleach. Does anyone else have any suggestions on what to do to ensure the fungus is eliminated without ruining the tissue?
> 
> Emily
> 
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> Message: 2
> Date: Thu, 24 Dec 2015 20:04:14 -0600
> From: Laurie Finch <riepopp2 at gmail.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Vegf.
> Message-ID:
>    <CAMjAh1Ss+dti1zPGp5va=_1iL3dX3HFwP44tAU0LtytB5O+D=A at mail.gmail.com>
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> 
> Hi Amos,
> 
> I have done vegf on a few different occasions for different species. For
> the biocare, I remember it needing a decloaker retrieval.  Not sure if you
> are using pt link or another retrieval type.
> 
> Laurie Popp,  HT/HTL ( ASCP ) cm
> 
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> End of Histonet Digest, Vol 145, Issue 24
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