[Histonet] Miniature arrays for control
SteveM at mcclainlab.com
Fri Dec 11 17:22:45 CST 2015
We have used miniature arrays for 9 years. We place both positive and negative controls on every IHC. Minimal design is one positive and one negative control.
To start make a simple one with positive and negative using a 2mm skin punch biopsy with plunger to cut out known tissue and mount together.
If you can't punch out tissue, or a valuable control is thin, then one can still make a miniature array.
Make an accordion or fan by cutting a 50 micron section and scrunch together.
Repeat with negative. Repeat with another positive. Wrap the whole thing in parafilm and Mount your layers on edge in paraffin. For example HHV8, KS marker, we have 5 controls 2 cases of KS, PG, hemangioma and normal skin.
Steve A. McClain, MD
> On Dec 10, 2015, at 13:20, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
> We are looking at the possible use of microarrays or tissue rolls for immuno controls on a routine basis. I would like to talk to people who are currently doing their immunos this way.
> We run about 120 slides a day with about 170 different primaries. Anyone with previous experience in this area (good or bad) who has any suggestions, I would be glad to hear about them.
> Thank you,
> Tim Vickroy
> Hershey Medical Center
> Penn State University
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