[Histonet] Mouse Brain Processing Help

[Catherine Simonson]

Sounds like insufficient processing and paraffin infiltration.
I deal with mouse tissue almost exclusively at my current facility.
Our processing schedule for sagital sections of brain is:

 70% ETOH 45 min
70% ETOH 45 min
95% ETOH 45 min
95% ETOH 45 min
100% ETOH 45 min
100% ETOH 45 min
100% ETOH 45 min
Xylene 45 min
Xylene 45 min
Xylene 45 min

Paraffin 45 min
Paraffin 45 min
Paraffin 45 min
Paraffin 45 min