[Histonet] Can Ice Crystal artifact pick up AEC substrate.
Lewis, Patrick
patrick.lewis at seattlechildrens.org
Wed Aug 5 13:04:23 CDT 2015
Hi Everyone,
I am seeing some red granular staining with my AEC substrate, and I am not sure if its real, or Ice crystal artifact, or debris from the slide directly.
It's in several areas and is consistent between duplicates.
So can I rule out slide debris?
How deep would Ice crystals go in a block. If I cut 10-15 7 uM sections deeper would I eliminate ice crystals from one slide to another?
I don't have any pictures yet, but I can try and post it when I do.
Patrick.
Patrick Lewis
Research Associate II Bench
Seattle Childrens Research Institute
206-884-1115
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