Re: [Histonet] Re: H. Pylori Testing

[wsimons <@t> athensgastro.com]

I prefer (and my pathologist) the Warthin Starry.  I performed that routinely, and affordably, on the Dako Artisan at my last employer.
At my current small lab we perform a Giemsa for h.pylori.   I've seen two cases of  H. heilmanii  in the last 2 years.  Both stained with Giemsa.  

When my lab expands I have the Artisan on my wishlist for both IHC and Warthin Starry.  Thank you everyone for
the comments.

Wanda K. Simons, HT (ASCP)
Athens Gastroenterology Association
3320 Old Jefferson Road, Bldg.400
Athens, GA 30607
www.histosearch.com/gsh/


>  -------Original Message-------
>  From: Garreyf <garreyf <@t> gmail.com>
>  To: Bob Richmond <rsrichmond <@t> gmail.com>
>  Cc: Histonet <@t> lists.utsouthwestern.edu <histonet <@t> lists.utsouthwestern.edu>
>  Subject: Re: [Histonet] Re: H. Pylori Testing
>  Sent: Apr 29 '15 14:21
>  
>  In the past when using giemsa stain,
>  I came across two human cases of very long helicobacter organisms.. I was stumped the first time since I had never seen one previously. I reflexed both to immuno and both were positive with the h pylori antibody. I assume they were both heilmani. I think it was called gastrospirillum in the past.I agree the immuno stain is much faster (easier)to look at.  I've never studied it but it's sensitivity is probably just a little better than giemsa. It's those cases with very few organisms that are more apt to  be missed using a non-immuno type of stain.  Agree about the contaminants as well .... we alway went on the seagull shaped morphology though.
>  
>  
>  Garrey
>  
>  Sent from my iPhone
>  
>  > On Apr 29, 2015, at 2:04 PM, Bob Richmond <rsrichmond <@t> gmail.com> wrote:
>  >
>  > Nancy Stedman observes:
>  >
>  >>> I believe IHC is more sensitive than the special stains too. One caveat
>  > for anyone who works with veterinary samples - the H. pylori antibodies are
>  > specific for H. pylori, so I have not found these antibodies to be helpful
>  > for evaluating other species with helicobacter-associated gastritis. One
>  > exception is the antibody made by Biocare which seems to stain some of the
>  > feline helicobacters, and maybe others too (have not tried).<<
>  >
>  > As far as I know, the only Helicobacter species other than H. pylori
>  > reported as a human pathogen is H. heilmanii - I've seen it exactly once,
>  > with a dye method - supposedly more common in Japan, often with a history
>  > of close association with cats. Supposedly H. heilmanii marks with the
>  > commercial IHC antibodies also.
>  >
>  > I don't think the data exist to show that IHC is more sensitive than the
>  > older dye methods, in terms of detecting clinical disease. As I noted
>  > before, the IHC is much faster for the pathologist to read. Also, many
>  > pathologists report any bacteria seen with dye methods as H. pylori,
>  > including the bacteria brought down by the endoscope from the oral cavity.
>  >
>  > Bob Richmond
>  > Samurai Pathologist
>  > Maryville TN
>  > _______________________________________________
>  > Histonet mailing list
>  > Histonet <@t> lists.utsouthwestern.edu
>  > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>  _______________________________________________
>  Histonet mailing list
>  Histonet <@t> lists.utsouthwestern.edu
>  http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>