[Histonet] Fwd: mouse tibia histology

[Shruti Shah]

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Begin forwarded message:

From: Shruti Shah <s.shah <@t> garvan.org.au<mailto:s.shah <@t> garvan.org.au>>
Date: 21 April 2015 7:28:07 pm AEST
To: Garrey Faller <garreyf <@t> gmail.com<mailto:garreyf <@t> gmail.com>>
Cc: "histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>" <histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>>
Subject: Re: [Histonet] (no subject)

Hi does any one doing mice tibia histology, we use to fix in formalin for 24 hours and transfer in 2and half weeks in 0.5M EDTA three change and process for 24 hours long protocol in automatic processor.
But I am facing problem with bone marrow shrinkage. If any one have idea for decalcification timing and solution can resolved this problem and keep bone marrow intact with bone.
Thank you in advance.

Regards,
Shruti

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On 21 Apr 2015, at 8:51 am, "Garrey Faller" <garreyf <@t> gmail.com<mailto:garreyf <@t> gmail.com>> wrote:

Here is the CAP checklist requirement:
ANP.21450
All  histochemical stains are of adequate quality, and daily controls are
demonstrated on each day of use for the tissue components or organism for
which they were designed.

Ray...you should call the CAP and ask for guidance on this.
My interpretation of this requirement is that it should be OK to use a
fungus from an orange peel. An orange peel fungus should have the same
staining characteristics as a candida or aspergillus etc.  Similarly a
bacteria is a bacteria. If you can produce a control that has both gram
positives and negatives, it should be OK. But, don't quote me on this.

Call the CAP for a definitive answer. I am interested in their response.
Garrey

On Sun, Apr 19, 2015 at 9:06 PM, <koellingr <@t> comcast.net<mailto:koellingr <@t> comcast.net>> wrote:

I asked about this in a different vein months ago.  Has anyone shown a
strawberry or ground meat or slim jim or orange peel as a bacteria/fungus
control used for diagnostics to an inspector inspecting the lab and was
there any comment from the inspector either positive or negative. Never
heard back anything.
Ray, Lake Forest Park, WA

----- Original Message -----

From: tjfinney2010 <@t> gmail.com<mailto:tjfinney2010 <@t> gmail.com>
To: histonet <@t> lists.utsouthwestern.edu<mailto:histonet <@t> lists.utsouthwestern.edu>
Sent: Sunday, April 19, 2015 5:24:53 PM
Subject: [Histonet] (no subject)

GMS controls
>From my understanding we can't use non human controls on patients. I
could be wrong, but you may want to look into it.

Happy Connecting.  Sent from my Sprint Phone.

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