[Histonet] controls to lengthy off topic

Tue Apr 21 08:49:21 CDT 2015

Hello Garrey, 
Curious myself, CAP contact info seems to be greyed out on website unless I officially log in and for now my concerns are with the Washington State Science and Engineering Fair for K-12 and golf game. 

(1) There are at least two phrases in the ANP.21450 which could be parsed out similar to the now famous "it depends on what the definition of is is". 
(2) Fortunate, I had micro groups around who could provide me with species specific Candida or Aspergillus or species and morphological identifiable gram positive or gram negative organisms so when I built the controls with fresh human tissue, as has been described several times on Histonet by others, I knew exactly what I was looking at. 
(3) It appears there may be tens to hundreds of thousands of "molds" and what is growing in orange peels or strawberries or cream cheese or bacteria in slim jims would be a total mystery but maybe that is OK. Yet, human pathogen or not? rare or common? stains appropriately or not according to what it REALLY is? 

I'm not saying the controls are wrong; they might be perfectly fine.  I'm just curious if anyone being inspected ever put a stained section of a slim jim on scope in front of a Pathologist from the inspecting agency and what was the reaction if any. 

Ray in Lake Forest Park, WA 
----- Original Message -----

From: "Garrey Faller" <garreyf <@t> gmail.com> 
To: koellingr <@t> comcast.net 
Cc: tjfinney2010 <@t> gmail.com, histonet <@t> lists.utsouthwestern.edu 
Sent: Monday, April 20, 2015 3:50:15 PM 
Subject: Re: [Histonet] (no subject) 

Here is the CAP checklist requirement: 
ANP.21450 
All  histochemical stains are of adequate quality, and daily controls are demonstrated on each day of use for the tissue components or organism for which they were designed. 

Ray...you should call the CAP and ask for guidance on this. 
My interpretation of this requirement is that it should be OK to use a fungus from an orange peel. An orange peel fungus should have the same staining characteristics as a candida or aspergillus etc.  Similarly a bacteria is a bacteria. If you can produce a control that has both gram positives and negatives, it should be OK. But, don't quote me on this.  

Call the CAP for a definitive answer. I am interested in their response. 
Garrey 

On Sun, Apr 19, 2015 at 9:06 PM, < koellingr <@t> comcast.net > wrote: 

I asked about this in a different vein months ago.  Has anyone shown a strawberry or ground meat or slim jim or orange peel as a bacteria/fungus control used for diagnostics to an inspector inspecting the lab and was there any comment from the inspector either positive or negative. Never heard back anything. 
Ray, Lake Forest Park, WA 

----- Original Message ----- 

From: tjfinney2010 <@t> gmail.com 
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Sunday, April 19, 2015 5:24:53 PM 
Subject: [Histonet] (no subject) 

GMS controls 
>From my understanding we can't use non human controls on patients. I could be wrong, but you may want to look into it. 

Happy Connecting.  Sent from my Sprint Phone. 

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