[Histonet] prevent wrinkles when cutting

[Podawiltz, Thomas]

Check you blade angle. Keep your blocks cold, change your blade often and just be patient. 


Tom Podawiltz HT (ASCP)
AP  Section Head 
LRGHealthcare
 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rachel M Gonzalez
Sent: Monday, April 20, 2015 12:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] prevent wrinkles when cutting

Hi

Thursday was the first time I ever used a microtome.... I move to a lab that does not have someone dedicated to cutting. I already miss her.

I have no problems getting ribbons of 10-30 sections long but the pieces are half the size of the original block. I am guessing they are wrinkling.
What am I doing wrong?

Thanks
Rachel
Senior Scientist....
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