[Histonet] RE: ORO tissue falling off

[Linda Prasad (SCHN)]

I Know fatty tissue is such a pain to cut. Bryan Llewellyn gave some really good techniques. Im going to try them out myself :)

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au


Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia

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-----Original Message-----
From: Caroline Miller [mailto:mills <@t> 3scan.com] 
Sent: Thursday, 16 April 2015 10:44 AM
To: Linda Prasad (SCHN)
Cc: Jo-Ann Bader, Ms.; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: ORO tissue falling off

+1 to Linda, but I have found no difference on overnight vs multiple days.

Fatty liver is hard to do on all counts! It is tough enough sometimes to get a decent section on the slide.

Thanks for the other suggestions, certainly something I would try in the future

Yours
Caroline

Caroline Miller (mills)
Director of Histology
3Scan, Inc
415-2187297

> On Apr 15, 2015, at 5:07 PM, Linda Prasad (SCHN) <linda.prasad <@t> health.nsw.gov.au> wrote:
> 
> Usually with the fatty tissues, I pick them up on superfrost slides and let it air dry  for 2-3 days at room temperature and then perform the ORO stains. So far they seem to stay on.
> 
> Linda Prasad | Senior Scientist | Histopathology
> t: (02) 9845 3306 | f: (02) 9845 3318 | e: 
> linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au
> 
> Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia 
> Locked Bag 4001, Westmead 2145, NSW Australia
> 
> ♲  Please consider the environment before printing this email.
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms.
> Sent: Thursday, 16 April 2015 1:40 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] ORO tissue falling off
> 
> We are having difficulty with a particulate set of very, very fatty 
> mouse livers.  The normal livers from this set stay on the slides the 
> fatty livers fall off.  We have used different types of charged slides 
> and we have even tried to drench the charged slides in Stay-On, dry 
> them and then put the frozen tissues on (despirate times call for 
> despirate measures).  No luck  Does anyone have any other ideas.  Help 
> Help
> 
> Jo-Ann  Bader
> Histology Coordinator
> Goodman Cancer Research Center
> 1600 Pine Ave. W,
> Room 312
> Montreal Quebec, H3A 1A3
> Email: jo-ann.bader <@t> mcgill.ca<mailto:jo-ann.bader <@t> mcgill.ca>
> Office Tel:  514-398-5647
> Lab:  Tel:  514-398-8270
> 
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This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.

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