[Histonet] Buffered formalin

[Julio Benavides]

Dear Bernice,

thanks a lot for your reply. The thing is that we received some samples 
fixed in that solution (buffered formalin made with PBS), and I was 
wondering if this could interfere with immunohistochemestry or normal HE 
staining.

BTW, writing from Spain, I guess commercial buffered formalin is cheap 
as well, but we have always made it in house.

cheers
julio

On 08/04/2015 18:08, Bernice Frederick wrote:
> Normally in my opinion, no. Usually the buffer is sodium phosphate monobasic and sodium phosphate dibasic. You can google the formula or as we get ours from Fisher it will tell you the mix. There is also millinog's formula and it is buffered but I don't use it so can't think of it off the top of my head.  A 5 gallon cube from Fisher is like 25$ here (I don't know where you are) so it's cheap to buy...
> Bernice
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick <@t> northwestern.edu
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Julio Benavides
> Sent: Wednesday, April 08, 2015 10:35 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Buffered formalin
>
> Hello,
>
> I was wondering in buffered formalin (for tissue samples fixation) could be prepared like follows:
>
> 100 ml PBS 10x
> 100 ml Formaldehyde 40%
> 800 ml Distilled water
>
> Thanks in advanced for your thoughts!!
>
> Regards
>
> Julio
>
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