[Histonet] Sihler stain for whole-mount nerves

[Harvey, Jennifer Lynn]

A fellow lab is asking if I know anyone doing a  Sihler stain<http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822894/> to visualize whole-mount nerves. The protocol calls for chloral hydrate, which they cannot get as a DEA class IV substance.
Better yet, are there alternatives to 1% w/v chloral hydrate/12% glycerol aqueous solutions for whole mount stainings? Looks like a very interesting stain but I can say I never heard of it. Any help would be appreciated.
Thanks



Jennifer Harvey, HT(ASCP) QIHC
Vanderbilt University Medical Center
Neuropathology Lab Supervisor
C-2309 Medical Center North
Nashville, TN  37232-2561
Phone: 615-343-0083
Fax: 615-343-7089