[Histonet] Re: Levai-Laczko stain

[fernandom.munoz <@t> usc.es]

1. 30% H2O2 5 min. under movement.
2. wash in water 2x
3. 5% Acetic acid 1 min.
4. wash in water 2x
5. solution 1 20 min.
6. wash in water 2x
7. solution 2, piece by piece
8. wash in water and dry.

Solution 1: 1 part Azur II (1%) + 1 part Methylen blue (1%) + 2 parts  
Na2CO3 (1%).
Solution 2: Pararosanillin (1%).
All solutions in distilled water. NEVER alcohol or acetone in plastic  
sections.

Fernando María Muñoz Guzón
Facultad de Veterinaria
Campus Universitario s/n
27002 Lugo (España)
Tfno: (+34)600940225