[Histonet] Kidney Stone Histology?

[Mike Andrews]

What Everyone Else Wrote.

They're stone (apatite; the kind I myself get) or hard crystals (uric
acid). They're very unfriendly to a microtome blade. Getting a thin section
of an apatite stone would require petrological thin-section techniques,
which won't be in the repertoire of the typical soft-tissue histo lab.
Think epoxy, abrasives, and time. Under a stereo scope, they can be
amazing: covered with sharp corners and edges, and all the corners and
edges have more corners and edges on them. For a big staghorn stone, a
diamond rock saw might be required. Uric acid crystals aren't particularly
better. But histo techniques pretty much don't apply.

On Fri, Apr 3, 2015 at 1:42 PM, Norton, Sally <
sally.norton <@t> seattlechildrens.org> wrote:

> The only thing we do with stones is send them to the Mayo clinic for
> chemical analysis.
>
> Sally Norton
> Seattle Children's
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jerry Ricks
> Sent: Friday, April 03, 2015 11:38 AM
> To: Histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Kidney Stone Histology?
>
> How the heck do I process and section kidney stones?  And what kind of
> stain do you like for them?
>
>
> Thanks
>
> Jerry Ricks
> Research Scientist
> University of Washington
> Department of Pathology
>
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-- 
73 de

Mike Andrews W5EGO