[Histonet] RE:Question about Formic acid decal and TRAP stain
koellingr <@t> comcast.net
koellingr <@t> comcast.net
Thu Apr 2 12:35:19 CDT 2015
Debra,
it appears most of the histology world disagrees with me but I stand by my post. If TRAP didn't work with formic acid in our hands, a major pharmaceutical treatment wouldn't be ready to help women with post-menopausal osteoporosis.
Ray
----- Original Message -----
From: "Sarah Mack" <Sarah_Mack <@t> urmc.rochester.edu>
To: histonet <@t> lists.utsouthwestern.edu
Sent: Thursday, April 2, 2015 10:21:21 AM
Subject: [Histonet] RE:Question about Formic acid decal and TRAP stain
In our hands we have never had TRAP success on Formic acid decalcified tissue.
Sarah Mack
University of Rochester Medical Center
Center for Musculoskeletal Research
Histology, Biochemistry, and Molecular Imaging Core
601 Elmwood Avenue
Box 665
Rochester, NY 14642
(585)-273-3901
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu [histonet-request <@t> lists.utsouthwestern.edu]
Sent: Thursday, April 02, 2015 1:02 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 137, Issue 3
Send Histonet mailing list submissions to
histonet <@t> lists.utsouthwestern.edu
To subscribe or unsubscribe via the World Wide Web, visit
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
or, via email, send a message with subject or body 'help' to
histonet-request <@t> lists.utsouthwestern.edu
You can reach the person managing the list at
histonet-owner <@t> lists.utsouthwestern.edu
When replying, please edit your Subject line so it is more specific
than "Re: Contents of Histonet digest..."
Today's Topics:
1. RE: Allowable temperature range (Linda Prasad (SCHN))
2. Positive Control for IF? (Paula Sicurello)
3. C3d? (Paula Sicurello)
4. Re: Positive Control for IF? (Mark Tarango)
5. Re: Positive Control for IF? (Jim Burchette)
6. RE: C3d? (Sebree Linda A)
7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt)
8. Question about Formic acid decal and TRAP stain (Debra Siena)
9. RE: Question about Formic acid decal and TRAP stain
(Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Wed, 1 Apr 2015 22:39:00 +0000
From: "Linda Prasad (SCHN)" <linda.prasad <@t> health.nsw.gov.au>
Subject: RE: [Histonet] Allowable temperature range
To: "'Tim H'" <thigginsht <@t> msn.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E88FF5 <@t> xmdb03.nch.kids>
Content-Type: text/plain; charset="utf-8"
If it's in formalin it can just stay at room temperature.
Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: https://urldefense.proofpoint.com/v2/url?u=http-3A__www.schn.health.nsw.gov.au&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=9Qb6_bEDCJKheqNhwxTb8wBpygzmxDAIDXa9byoCD2k&e=
Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia
Locked Bag 4001, Westmead 2145, NSW Australia
♲ Please consider the environment before printing this email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim H
Sent: Thursday, 2 April 2015 1:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Allowable temperature range
What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage?
Any ideas?
Tim
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
*********************************************************************************
This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.
Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.
This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.
*********************************************************************************
------------------------------
Message: 2
Date: Wed, 1 Apr 2015 16:12:22 -0700
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: [Histonet] Positive Control for IF?
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAPSjddaNxLOoPhtQ0y4=aHU2qg7nnZgBhWFWoMFVfyFnGYMyRw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Good Afternoon Netters,
Since we went down the path of hot dogs and Slim Jim's as positive controls
for FFPE stains, I was wondering.....
Is there a source, like a hot dog or piece of steak, that could be a
positive control for immunofluorescence C4d?
What I'd really like to find is some food or food product (our positive
patient biopsies for the frozen IF are teeny-tiny) that is positive for the
whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture.
Please send your suggestions my way.
Thanks in advance,
Paula :-)
------------------------------
Message: 3
Date: Wed, 1 Apr 2015 16:14:16 -0700
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: [Histonet] C3d?
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CAPSjddYaAk+kz1mvw+a_e9YdARaZgw9-fnUsvvXucfcA7k80kw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Hello Again My Dear Netters,
One of our pathologists was wondering if anyone out in Histoland is
performing a C3d stain?
If so what type: IHC, IF?
Thanks oodles!
Paula :-)
------------------------------
Message: 4
Date: Wed, 1 Apr 2015 16:16:54 -0700
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Positive Control for IF?
To: Paula Sicurello <patpxs <@t> gmail.com>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CACJwj1KEaQTqBsSp+tGznrxmEwbPmBuByXOHcajbeLpd=_31Bw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
It is April 1st, isn't it?
On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello <patpxs <@t> gmail.com> wrote:
> Good Afternoon Netters,
>
> Since we went down the path of hot dogs and Slim Jim's as positive controls
> for FFPE stains, I was wondering.....
>
> Is there a source, like a hot dog or piece of steak, that could be a
> positive control for immunofluorescence C4d?
>
> What I'd really like to find is some food or food product (our positive
> patient biopsies for the frozen IF are teeny-tiny) that is positive for the
> whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the
> picture.
>
> Please send your suggestions my way.
>
> Thanks in advance,
>
> Paula :-)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
>
------------------------------
Message: 5
Date: Wed, 1 Apr 2015 20:16:29 -0500
From: Jim Burchette <jburch01 <@t> gmail.com>
Subject: Re: [Histonet] Positive Control for IF?
To: Paula Sicurello <patpxs <@t> gmail.com>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CANDy+MeDmBzpGWobA68XwAeoXoMGtyA5Ts5B32MALMdi0dWBQw <@t> mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Paula, does your institution perform autopsies? If so, have the staff
notify you when there is a lupus nephritis case. Have them send you
fresh renal cortex. Cut up the tissue into small cubes and freeze for later
use as IF control. Run your battery of Ig's, compliment and light chains.
Most should have some reactivity.
You might be able to use a rejected kidney for C4d if it isn't to necrotic.
Transplant heart and liver can also be used for rejection antibodies.
JB
On Wednesday, April 1, 2015, Paula Sicurello <patpxs <@t> gmail.com> wrote:
> Good Afternoon Netters,
>
> Since we went down the path of hot dogs and Slim Jim's as positive controls
> for FFPE stains, I was wondering.....
>
> Is there a source, like a hot dog or piece of steak, that could be a
> positive control for immunofluorescence C4d?
>
> What I'd really like to find is some food or food product (our positive
> patient biopsies for the frozen IF are teeny-tiny) that is positive for the
> whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the
> picture.
>
> Please send your suggestions my way.
>
> Thanks in advance,
>
> Paula :-)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
>
--
Jim Burchette
"Fly Fishing Bum" *<'(((><*
------------------------------
Message: 6
Date: Thu, 2 Apr 2015 12:25:05 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] C3d?
To: 'Paula Sicurello' <patpxs <@t> gmail.com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF121997 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="utf-8"
Cleveland Clinic does it by IHC and I think also by IF.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Wednesday, April 01, 2015 6:14 PM
To: HistoNet
Subject: [Histonet] C3d?
Hello Again My Dear Netters,
One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain?
If so what type: IHC, IF?
Thanks oodles!
Paula :-)
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
------------------------------
Message: 7
Date: Thu, 2 Apr 2015 14:13:49 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] interface between Bond and Cerner CoPath Plus
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C3601159C5EFA <@t> PEITHA.wad.pa-ucl.com>
Content-Type: text/plain; charset="us-ascii"
Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? How does it work? Cost?
Thank you
Nancy Schmitt MLT, HT(ASCP)
NOTICE: This email may contain legally privileged information. The information
is for the use of only the intended recipient(s) even if addressed
incorrectly. If you are not the intended recipient, please notify the sender
that you have received it in error and then delete it along with any
attachments. Thank you.
------------------------------
Message: 8
Date: Thu, 2 Apr 2015 16:00:25 +0000
From: Debra Siena <DSiena <@t> statlab.com>
Subject: [Histonet] Question about Formic acid decal and TRAP stain
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<BY2PR07MB1055C4721C256106D8593B3DCF20 <@t> BY2PR07MB105.namprd07.prod.outlook.com>
Content-Type: text/plain; charset="us-ascii"
Hi Histonetters,
I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help.
Debbie Siena
dsiena <@t> statlab.com <mailto:bbrooke <@t> statlab.com%7C> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=-xV-E9SCM5fSHPL_V_8Xc1Hoe1aXMvZtCexLeO2O5Mk&e= >
------------------------------
Message: 9
Date: Thu, 2 Apr 2015 10:10:11 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: Question about Formic acid decal and TRAP
stain
To: Debra Siena <DSiena <@t> statlab.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE019C79F17FFC <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Debbie
In our hands it has not worked on formic acid decaled samples, EDTA decal only.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.premierlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=F3th4LJ6QUaggfgVvpdB5XB0_s-ZkCp_YEJKag4d-Ic&e=
March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debra Siena
Sent: Thursday, April 02, 2015 10:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question about Formic acid decal and TRAP stain
Hi Histonetters,
I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help.
Debbie Siena
dsiena <@t> statlab.com <mailto:bbrooke <@t> statlab.com%7C> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=-xV-E9SCM5fSHPL_V_8Xc1Hoe1aXMvZtCexLeO2O5Mk&e= >
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
------------------------------
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
End of Histonet Digest, Vol 137, Issue 3
****************************************
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
More information about the Histonet
mailing list