[Histonet] RE:Question about Formic acid decal and TRAP stain
Mack, Sarah
Sarah_Mack <@t> urmc.rochester.edu
Thu Apr 2 12:21:21 CDT 2015
In our hands we have never had TRAP success on Formic acid decalcified tissue.
Sarah Mack
University of Rochester Medical Center
Center for Musculoskeletal Research
Histology, Biochemistry, and Molecular Imaging Core
601 Elmwood Avenue
Box 665
Rochester, NY 14642
(585)-273-3901
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Subject: Histonet Digest, Vol 137, Issue 3
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Today's Topics:
1. RE: Allowable temperature range (Linda Prasad (SCHN))
2. Positive Control for IF? (Paula Sicurello)
3. C3d? (Paula Sicurello)
4. Re: Positive Control for IF? (Mark Tarango)
5. Re: Positive Control for IF? (Jim Burchette)
6. RE: C3d? (Sebree Linda A)
7. interface between Bond and Cerner CoPath Plus (Nancy Schmitt)
8. Question about Formic acid decal and TRAP stain (Debra Siena)
9. RE: Question about Formic acid decal and TRAP stain
(Elizabeth Chlipala)
----------------------------------------------------------------------
Message: 1
Date: Wed, 1 Apr 2015 22:39:00 +0000
From: "Linda Prasad (SCHN)" <linda.prasad <@t> health.nsw.gov.au>
Subject: RE: [Histonet] Allowable temperature range
To: "'Tim H'" <thigginsht <@t> msn.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <1217DDB3D7DE5E418E3D560A268EABD0E0E88FF5 <@t> xmdb03.nch.kids>
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If it's in formalin it can just stay at room temperature.
Linda Prasad | Senior Scientist | Histopathology
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Tim H
Sent: Thursday, 2 April 2015 1:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Allowable temperature range
What is the allowable temperature range for a histology specimen after collection in formalin for shipping and storage?
Any ideas?
Tim
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Message: 2
Date: Wed, 1 Apr 2015 16:12:22 -0700
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: [Histonet] Positive Control for IF?
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
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Good Afternoon Netters,
Since we went down the path of hot dogs and Slim Jim's as positive controls
for FFPE stains, I was wondering.....
Is there a source, like a hot dog or piece of steak, that could be a
positive control for immunofluorescence C4d?
What I'd really like to find is some food or food product (our positive
patient biopsies for the frozen IF are teeny-tiny) that is positive for the
whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the picture.
Please send your suggestions my way.
Thanks in advance,
Paula :-)
------------------------------
Message: 3
Date: Wed, 1 Apr 2015 16:14:16 -0700
From: Paula Sicurello <patpxs <@t> gmail.com>
Subject: [Histonet] C3d?
To: HistoNet <histonet <@t> lists.utsouthwestern.edu>
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Hello Again My Dear Netters,
One of our pathologists was wondering if anyone out in Histoland is
performing a C3d stain?
If so what type: IHC, IF?
Thanks oodles!
Paula :-)
------------------------------
Message: 4
Date: Wed, 1 Apr 2015 16:16:54 -0700
From: Mark Tarango <marktarango <@t> gmail.com>
Subject: Re: [Histonet] Positive Control for IF?
To: Paula Sicurello <patpxs <@t> gmail.com>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CACJwj1KEaQTqBsSp+tGznrxmEwbPmBuByXOHcajbeLpd=_31Bw <@t> mail.gmail.com>
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It is April 1st, isn't it?
On Wed, Apr 1, 2015 at 4:12 PM, Paula Sicurello <patpxs <@t> gmail.com> wrote:
> Good Afternoon Netters,
>
> Since we went down the path of hot dogs and Slim Jim's as positive controls
> for FFPE stains, I was wondering.....
>
> Is there a source, like a hot dog or piece of steak, that could be a
> positive control for immunofluorescence C4d?
>
> What I'd really like to find is some food or food product (our positive
> patient biopsies for the frozen IF are teeny-tiny) that is positive for the
> whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the
> picture.
>
> Please send your suggestions my way.
>
> Thanks in advance,
>
> Paula :-)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
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>
------------------------------
Message: 5
Date: Wed, 1 Apr 2015 20:16:29 -0500
From: Jim Burchette <jburch01 <@t> gmail.com>
Subject: Re: [Histonet] Positive Control for IF?
To: Paula Sicurello <patpxs <@t> gmail.com>
Cc: HistoNet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<CANDy+MeDmBzpGWobA68XwAeoXoMGtyA5Ts5B32MALMdi0dWBQw <@t> mail.gmail.com>
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Paula, does your institution perform autopsies? If so, have the staff
notify you when there is a lupus nephritis case. Have them send you
fresh renal cortex. Cut up the tissue into small cubes and freeze for later
use as IF control. Run your battery of Ig's, compliment and light chains.
Most should have some reactivity.
You might be able to use a rejected kidney for C4d if it isn't to necrotic.
Transplant heart and liver can also be used for rejection antibodies.
JB
On Wednesday, April 1, 2015, Paula Sicurello <patpxs <@t> gmail.com> wrote:
> Good Afternoon Netters,
>
> Since we went down the path of hot dogs and Slim Jim's as positive controls
> for FFPE stains, I was wondering.....
>
> Is there a source, like a hot dog or piece of steak, that could be a
> positive control for immunofluorescence C4d?
>
> What I'd really like to find is some food or food product (our positive
> patient biopsies for the frozen IF are teeny-tiny) that is positive for the
> whole host of IF stains: IgG, IgA, IgM, Kappa, Lambda, you get the
> picture.
>
> Please send your suggestions my way.
>
> Thanks in advance,
>
> Paula :-)
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu <javascript:;>
> https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=OUpH8OsAE5aX_ZTdjsfvOwqMMBDeESpHHBEfWaX3Fzg&e=
>
--
Jim Burchette
"Fly Fishing Bum" *<'(((><*
------------------------------
Message: 6
Date: Thu, 2 Apr 2015 12:25:05 +0000
From: Sebree Linda A <LSebree <@t> uwhealth.org>
Subject: RE: [Histonet] C3d?
To: 'Paula Sicurello' <patpxs <@t> gmail.com>, HistoNet
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<77DD817201982748BC67D7960F2F76AF121997 <@t> UWHC-MBX12.uwhis.hosp.wisc.edu>
Content-Type: text/plain; charset="utf-8"
Cleveland Clinic does it by IHC and I think also by IF.
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory, Rm A4/204-3224
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Paula Sicurello
Sent: Wednesday, April 01, 2015 6:14 PM
To: HistoNet
Subject: [Histonet] C3d?
Hello Again My Dear Netters,
One of our pathologists was wondering if anyone out in Histoland is performing a C3d stain?
If so what type: IHC, IF?
Thanks oodles!
Paula :-)
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------------------------------
Message: 7
Date: Thu, 2 Apr 2015 14:13:49 +0000
From: Nancy Schmitt <Nancy_Schmitt <@t> pa-ucl.com>
Subject: [Histonet] interface between Bond and Cerner CoPath Plus
To: "'histonet <@t> lists.utsouthwestern.edu'"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<906B4DA90ED1DB4DB6C7E94D7CEE6C3601159C5EFA <@t> PEITHA.wad.pa-ucl.com>
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Does anyone currently have an interface between the Leica Bond stainer and Cerner CoPath plus? How does it work? Cost?
Thank you
Nancy Schmitt MLT, HT(ASCP)
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Message: 8
Date: Thu, 2 Apr 2015 16:00:25 +0000
From: Debra Siena <DSiena <@t> statlab.com>
Subject: [Histonet] Question about Formic acid decal and TRAP stain
To: "histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
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Hi Histonetters,
I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help.
Debbie Siena
dsiena <@t> statlab.com <mailto:bbrooke <@t> statlab.com%7C> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=-xV-E9SCM5fSHPL_V_8Xc1Hoe1aXMvZtCexLeO2O5Mk&e= >
------------------------------
Message: 9
Date: Thu, 2 Apr 2015 10:10:11 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: Question about Formic acid decal and TRAP
stain
To: Debra Siena <DSiena <@t> statlab.com>,
"histonet <@t> lists.utsouthwestern.edu"
<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<14E2C6176416974295479C64A11CB9AE019C79F17FFC <@t> SBS2K8.premierlab.local>
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Debbie
In our hands it has not worked on formic acid decaled samples, EDTA decal only.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
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March 10, 2014 is Histotechnology Professionals Day
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Debra Siena
Sent: Thursday, April 02, 2015 10:00 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Question about Formic acid decal and TRAP stain
Hi Histonetters,
I have a question to ask if you don't mind. Can TRAP Histochemical staining be performed after decalcifying with formic acid? Any tricks of the trade, etc? If anyone has any experience or references that they could point me to, I would greatly appreciate it. Thanks in advance for your help.
Debbie Siena
dsiena <@t> statlab.com <mailto:bbrooke <@t> statlab.com%7C> | https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=6M7YFVJIgiwHHPZ8-KqMID94w_dbq7fnrJzokYbVuxg&e= <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.statlab.com_&d=AwIDaQ&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=KnPSEWHFntXYp-Xo0ibYtyMEBbK1UL1yi6Dm03IvZLE&s=-xV-E9SCM5fSHPL_V_8Xc1Hoe1aXMvZtCexLeO2O5Mk&e= >
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