[Histonet] Sihler staining

[John Kiernan]

No first-hand knowledge, unfortunately, but in addition to the five references in the 2005 Histonet post that you mentioned -
http://lists.utsouthwestern.edu/mailman/htdig/histonet/2005-November/018700.html - 
there is a more recent review with plenty of technical details and photos:
Mu, L. and Sanders, I. (2010). Sihler's whole mount nerve staining technique: a review. Biotechnic & Histochemistry 85:19-42. The online version has impressive colour photos; the print version has only B&W.
The corresponding author is LMU <@t> HUMED.COM (Hackensack Univ., NJ). I don't know if he's still there. 
Hope this helps.
John Kiernan
London, Canada
= = =
On 01/04/15, Caroline Miller  <mills <@t> 3scan.com> wrote:
> Hi Histonet,
> 
> I just started working for a small start up in San Francisco called 3scan,
> after 18 years in clinical (human) and academia (animal tissues). We have a
> microscope that both sections and images at the same time. This means that
> my job is to get stain into whole mount tissues prior to sectioning. We
> found the Sihler staining technique for peripheral nerves and have been
> getting it to work well in animal organs that were taken directly from the
> animal and fixed.
> 
> We are now trying embalmed human tissue, which is our final target. We had
> two samples, the first one came out very ragged and I don't think it had
> any nerve tissue left in it after the maceration. It was really hard to
> know where the end point is, it was so subjective, especially when looking
> at unfamiliar tissues. I am thinking the first sample came out so ragged
> because we did not put the sample into formaldehyde prior to maceration
> because it was embalmed and we thought that was enough.
> 
> We have one more sample that is now dissected and sat in unbuffered 4%
> formaldehyde. *I wanted to see if anyone familiar with the technique was
> prepared to have a conversation with me about the end points of each stage,
> *We have totally read and digested all of John's suggestions from this page:
> http://lists.utsouthwestern.edu/mailman/htdig/histonet//2005-November/018700.html
> plus many others, but it would be great to talk to someone first hand.
> 
> I reached out to Mu and Sanders, but they are no longer at that institution
> and I can't find them. Or the other person who posted on Sihler to histonet
> in 2005 - *Maria Mejia* maria <@t> ski.org. Their email address bounces
> now
> <histonet%40lists.utsouthwestern.edu?Subject=%5BHistonet%5D%20Sihler%27s%20stain&In-Reply-To=>
> 
> Is there anyone out there with first hand knowledge I can have a chat with?
> 
> Thanks all,
> 
> mills aka Caroline :)
> 
> 
> -- 
> Caroline Miller
> Director of Histology
> 3Scan.com
> 415 2187297
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