[Histonet] At long last, a Murine CD4 monoclonal antibody that works on FFPE tissues

[gayle callis]

Dear Histonetters, 

 

This antibody is going to be very welcome for those working with NBF fixed
paraffin embedded murine tissue.    It is a rat antiMouse CD4  for FFPE
tissue.   For those interested go to Affymetrix/eBioscience, Rat antiMouse
CD4, clone 4SM95 (L3T4/Ly-4,  Catalog number 14-9766.   It also comes
conjugated to the company's eFluor 570, equivalent to Alexa 555 although one
could do their own conjugation to their favorite Alexa or DyLight dye.   

 

Now all we need is a mouse CD8 that works on FFPE for total happiness since
we often do these two antibodies in tandem as a double IF stain or on
adjacent sections for enzyme IHC.    

 

Take care

 

Gayle M. Callis

HTL/HT/MT(ASCP)