[Histonet] mounting media for immunofluorescence

[Tony Henwood (SCHN)]

Hi Christina,

Espada et al (2005) have shown that fluorochromes are very well preserved after dehydration (or simply air drying) followed by permanent mounting in non-aqueous synthetic media, particularly DePeX. They also found that fluorescence had not faded after one year of storage at room temperature.
(Espada, J., Juarranz, Á., Galaz, S., Cañete, M., Villanueva, Á., Pacheco, M., & Stockert, J. C. (2005). Non-aqueous permanent mounting for immunofluorescence microscopy. Histochemistry and cell biology, 123(3), 329-334)

This might be worth a try

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Christina Kreutzer
Sent: Tuesday, 16 September 2014 1:12 AM
To: <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] mounting media for immunofluorescence

Hello folks,
I am wondering which mounting medium für IF you are using in your daily routine.
We are using Prolong antifade gold, but it is really really really expensive and I was wondering if there is a good alternative. I just ordered a sample of the Dako Fluorescence Mounting Medium so we can try that , but we are a little bit concerned about how long lasting the fluorescence signal would be.

Well, what are your experiences so far.

All the best
Christina
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