[Histonet] mounting media for immunofluorescence

James Watson JWatson <@t> gnf.org
Mon Sep 15 12:57:24 CDT 2014

We have used a hardmount Thermo "Immumount"  Cat# 9990402.  It is the only hardmount we tested that gave good focus using the NanoZoomer scanner.  No need to seal with nail polish,  saving a lot of time.  We just re-scanned slides that were stained 6 months ago and still had great fluorescence.  We use life Technologies "Alexa Fluor" secondary antibodies.

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel    858-332-4647
Fax   858-812-1915
jwatson <@t> gnf.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Segovia, Claudia
Sent: Monday, September 15, 2014 8:36 AM
To: Christina Kreutzer
Cc: <histonet <@t> lists.utsouthwestern.edu>
Subject: Re: [Histonet] mounting media for immunofluorescence

Hi Christina,

We use DPX. It has worked very well for us. I am not sure about the cost though, but anything bought in kits can get really pricy. DPX comes in 500 ml.

Claudia N. Segovia
Senior Neurohistologist
Antibody Specialist
NeuroScience Associates
10915 Lake Ridge Drive
Knoxville, TN  37934
csegovia <@t> nsalabs.com

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> On Sep 15, 2014, at 11:21 AM, "Christina Kreutzer" <christina.kreutzer01 <@t> gmail.com> wrote:
> Hello folks,
> I am wondering which mounting medium für IF you are using in your 
> daily routine.
> We are using Prolong antifade gold, but it is really really really 
> expensive and I was wondering if there is a good alternative. I just 
> ordered a sample of the Dako Fluorescence Mounting Medium so we can 
> try that , but we are a little bit concerned about how long lasting 
> the fluorescence signal would be.
> Well, what are your experiences so far.
> All the best
> Christina
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