[Histonet] RE: MOHS

[Keyser Gerald T]

Embedding:

I use a large, solid aluminum block that I keep cool in my cryostat. It's approx. 9 in (length) x 4 in (width) x 3 in (depth). I just stick the epidermal edge to the block and flatten it out so all margins are in contact with the freezing surface. Drop some freezing medium on it and attach it to a specimen holder. It's good to go. 

A large, thick, removable metal block as a freezing surface should be standard equipment for all cryostats. 

Sectioning: 

Having a liquid nitrogen gun to selectively freeze fatty constituents in the specimen is a good idea. 

After I collect the specimens on a slide I check it with a microscope with the light source turned on very low. That allows the tech to see if the epidermis is completely represented in the specimen. Other techs I work with do it a bit differently. But, the results I get from a quick examination under a microscope is excellent.  

 Gerald Keyser

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A
Sent: Thursday, September 11, 2014 5:24 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MOHS

We are looking at bringing MOHS in-house for a new physician.  We already do a great deal of frozen sections, so we are set up for cryotomy and staining, however, I am in need of a good procedure for embedding, cryotomy etc.
Thank you,
Lacie

Lacie Algeo, HTL (ASCP) MBCM
Histology Supervisor
Providence Sacred Heart Medical Center Laboratory
101 W 8th Avenue
L-2
Spokane, WA 99204
509-474-4418
FAX 509-474-2052
lacie.algeo <@t> providence.org<mailto:lacie.algeo <@t> providence.org>


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