[Histonet] Re: Mouse on Mouse Staining

Michael Thompson mike <@t> dbiosys.com
Mon Sep 8 17:56:55 CDT 2014

Diagnostic Biosystems has MOM kit.

Look at website below

Email me for a sample

Michael Thompson
Dir of Sales
Diagnostic BioSystems
"IHC Made Affordable"

Sent from my Verizon Wireless 4G LTE DROID

"anna.c.hughes" <anna.c.hughes <@t> gsk.com> wrote:

Hi John!

 GeneTex makes a Rabbit Poly against mouse for HDAC1 :
http://www.genetex.com/HDAC1-antibody-GTX100513.html   and LSBio makes a
Rabbit Poly against Nirtrotyrosine :
I always look for alternative hosts for antibodies rather than do something
like mouse on mouse first.  I have also had very good luck with with LSBio
and GeneTex antibodies.  Good Luck!

Anna Hughes

*Anna C. Hughes, BBA, BS, HTL (ASCP), QIHC*

Senior Scientist,  Morphologic Pathology



anna.c.hughes <@t> gsk.com

On Thursday, July 24, 2014 9:11:29 AM UTC-4, John wrote:

>  Good Morning,
> I have avoided for the last few years doing IHC with mouse on mouse
> antibodies, but have been requested to do so at this time. I have two
> antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up,
> however the staining pattern in my tissue does not seem to be revealing the
> accurate staining pattern. I was hoping someone in the research community
> has worked with these antibodies, even if from other vendors and would be
> willing to discuss further with me. Thanks!!!
> Kind Regards!
> John J Shelley
> Research Specialist, Histology Core Facility
> *Sanford-Burnham Medical Research Institute at Lake Nona*

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