[Histonet] Re: Mouse on Mouse Staining

[anna.c.hughes]

Hi John! 
 
 GeneTex makes a Rabbit Poly against mouse for HDAC1 : 
http://www.genetex.com/HDAC1-antibody-GTX100513.html   and LSBio makes a 
Rabbit Poly against Nirtrotyrosine : 
http://www.lsbio.com/antibodies/anti-nitrotyrosine-antibody-biotin-ihc-wb-western-elisa-ls-c299307/309222   
I always look for alternative hosts for antibodies rather than do something 
like mouse on mouse first.  I have also had very good luck with with LSBio 
and GeneTex antibodies.  Good Luck!
 
Anna Hughes

*Anna C. Hughes, BBA, BS, HTL (ASCP), QIHC*

Senior Scientist,  Morphologic Pathology 

GlaxoSmithKline

610-239-5771

anna.c.hughes <@t> gsk.com

On Thursday, July 24, 2014 9:11:29 AM UTC-4, John wrote:

>  Good Morning,
>
>  
>
> I have avoided for the last few years doing IHC with mouse on mouse 
> antibodies, but have been requested to do so at this time. I have two 
> antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up, 
> however the staining pattern in my tissue does not seem to be revealing the 
> accurate staining pattern. I was hoping someone in the research community 
> has worked with these antibodies, even if from other vendors and would be 
> willing to discuss further with me. Thanks!!! 
>
>  
>
> Kind Regards!
>
>  
>
> John J Shelley
>
> Research Specialist, Histology Core Facility
>
> *Sanford-Burnham Medical Research Institute at Lake Nona*
>
>  
>