[Histonet] Neutrophil staining

James Watson JWatson <@t> gnf.org
Mon Sep 8 13:04:13 CDT 2014

We have been using this Rat antibody in mouse tissue with both fluorescence and Chromogen detection,  The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.





James Watson HT  ASCP

GNF  Genomics Institute of the Novartis Research Foundation

Scientific Technical Leader II, Histology

Tel    858-332-4647

Fax   858-812-1915

jwatson <@t> gnf.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Saturday, September 06, 2014 2:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining

Hello All,

I am looking to  stain neutrophils using special stains and antibodies in mice tissue.  I wanted your opinion about the best methods to do so.  I am thinking of using the may Grunwald giemsa and T-blue for specials and myeloperoxidase and Ly6G for the antibodies.

What are your favorite special stains and antibodies to stain for neutrophils?

Best regards

Hans B Snyder


60 Prescott Street

Worcester, MA 01605


hans <@t> histologistics.com<mailto:hans <@t> histologistics.com>


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