AW: [Histonet] Neutrophil staining
gu.lang <@t> gmx.at
Mon Sep 8 08:45:23 CDT 2014
What about PAS staining? Granulozyte granula are PAS positiv.
Von: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Connolly, Brett M
Gesendet: Montag, 08. September 2014 15:30
An: Hans B Snyder; histonet <@t> lists.utsouthwestern.edu
Betreff: RE: [Histonet] Neutrophil staining
We had very good staining using Ly-6B.2 from Serotec (MCA771) on C57B6 mice, but needed to switch to Ly-6G for CH3 mice. The MCA771 datasheet tells you which strains react with that antibody.
Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Hans B Snyder
Sent: Saturday, September 06, 2014 5:43 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Neutrophil staining
I am looking to stain neutrophils using special stains and antibodies in mice tissue. I wanted your opinion about the best methods to do so. I am thinking of using the may Grunwald giemsa and T-blue for specials and myeloperoxidase and Ly6G for the antibodies.
What are your favorite special stains and antibodies to stain for neutrophils?
Hans B Snyder
60 Prescott Street
Worcester, MA 01605
hans <@t> histologistics.com
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