[Histonet] Re: Neutrophil staining

[Patsy Ruegg]

used to cae back in the day and it picked up granules perfectly and was very easy to do

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net


> Date: Sun, 7 Sep 2014 21:04:06 -0400
> From: rsrichmond <@t> gmail.com
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] Re: Neutrophil staining
> 
> Hans B Snyder at Histologistics in Worcester, Massachusetts asks:
> 
> >>I am looking to stain neutrophils using special stains and antibodies in
> mice tissue. I wanted your opinion about the best methods to do so. I am
> thinking of using the May-Grünewald Giemsa and T-blue for specials and
> myeloperoxidase and Ly6G for the antibodies.<<
> 
> I haven't done it and don't know if it works on mice, but histochemical
> staining for chloroacetate esterase is extremely simple to do, and supposed
> to be quite specific.
> 
> Bob Richmond
> Samurai Pathologist
> Maryville, Tennessee
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