[Histonet] Color in Sections with Adipose Tissue?

[jerry sedgewick]

Thanks, Colleen...Got it!


On 9/4/2014 1:55 PM, Colleen Forster wrote:
> When I cut adipose tissues Jerry, that have been fixed and processed 
> you will get a slide that appears clear or like tissue is missing in 
> between the vessels. If you look under the scope you will see fine 
> cell membranes that outline the fat cell. The fat dissolves during 
> processing leaving only the membrane and vasculature. If they want to 
> see the actual fat it has to be done by frozen section.
>
> Does this make sense?
>
> C
>
>
> On Thu, Sep 4, 2014 at 12:52 PM, jerry sedgewick 
> <jerrysedgewick <@t> gmail.com <mailto:jerrysedgewick <@t> gmail.com>> wrote:
>
>     I've observed images on a computer screen with a person next to me
>     insisting that the adipose tissue in H&E stained tissue has a
>     color versus areas with no tissue (open areas).  Am I missing
>     something, or do others see either a slight discoloration in
>     adipose tissue, or a slightly different color, versus areas
>     without tissue?  I suspect it depends on section thickness, but
>     given a 5 - 8 micron section it seems that whatever tissue might
>     have been present would be scraped away with the blade.
>
>     Jerry Sedgewick
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