[Histonet] Color in Sections with Adipose Tissue?
jerrysedgewick <@t> gmail.com
Thu Sep 4 14:20:26 CDT 2014
Thanks, Colleen...Got it!
On 9/4/2014 1:55 PM, Colleen Forster wrote:
> When I cut adipose tissues Jerry, that have been fixed and processed
> you will get a slide that appears clear or like tissue is missing in
> between the vessels. If you look under the scope you will see fine
> cell membranes that outline the fat cell. The fat dissolves during
> processing leaving only the membrane and vasculature. If they want to
> see the actual fat it has to be done by frozen section.
> Does this make sense?
> On Thu, Sep 4, 2014 at 12:52 PM, jerry sedgewick
> <jerrysedgewick <@t> gmail.com <mailto:jerrysedgewick <@t> gmail.com>> wrote:
> I've observed images on a computer screen with a person next to me
> insisting that the adipose tissue in H&E stained tissue has a
> color versus areas with no tissue (open areas). Am I missing
> something, or do others see either a slight discoloration in
> adipose tissue, or a slightly different color, versus areas
> without tissue? I suspect it depends on section thickness, but
> given a 5 - 8 micron section it seems that whatever tissue might
> have been present would be scraped away with the blade.
> Jerry Sedgewick
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