[Histonet] Color in Sections with Adipose Tissue?

[jerry sedgewick]

Oh yeah, didn't think about frozen vs paraffin section since I'm an 
imaging guy.  If it were frozen, would some remnant of the adipose 
tissue remain after sectioning? Thanks!

On 9/4/2014 1:02 PM, jsjurczak <@t> comcast.net wrote:
>
> Are you talking frozens here?
>
>
>
> ------------------------------------------------------------------------
> *From: *"jerry sedgewick" <jerrysedgewick <@t> gmail.com>
> *To: *"Histonet <@t> lists.utsouthwestern.edu" 
> <histonet <@t> lists.utsouthwestern.edu>
> *Sent: *Thursday, September 4, 2014 12:52:30 PM
> *Subject: *[Histonet] Color in Sections with Adipose Tissue?
>
> I've observed images on a computer screen with a person next to me
> insisting that the adipose tissue in H&E stained tissue has a color
> versus areas with no tissue (open areas).  Am I missing something, or do
> others see either a slight discoloration in adipose tissue, or a
> slightly different color, versus areas without tissue?  I suspect it
> depends on section thickness, but given a 5 - 8 micron section it seems
> that whatever tissue might have been present would be scraped away with
> the blade.
>
> Jerry Sedgewick
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>