[Histonet] Color in Sections with Adipose Tissue?

jerry sedgewick jerrysedgewick <@t> gmail.com
Thu Sep 4 13:59:06 CDT 2014

Oh yeah, didn't think about frozen vs paraffin section since I'm an 
imaging guy.  If it were frozen, would some remnant of the adipose 
tissue remain after sectioning? Thanks!

On 9/4/2014 1:02 PM, jsjurczak <@t> comcast.net wrote:
> Are you talking frozens here?
> ------------------------------------------------------------------------
> *From: *"jerry sedgewick" <jerrysedgewick <@t> gmail.com>
> *To: *"Histonet <@t> lists.utsouthwestern.edu" 
> <histonet <@t> lists.utsouthwestern.edu>
> *Sent: *Thursday, September 4, 2014 12:52:30 PM
> *Subject: *[Histonet] Color in Sections with Adipose Tissue?
> I've observed images on a computer screen with a person next to me
> insisting that the adipose tissue in H&E stained tissue has a color
> versus areas with no tissue (open areas).  Am I missing something, or do
> others see either a slight discoloration in adipose tissue, or a
> slightly different color, versus areas without tissue?  I suspect it
> depends on section thickness, but given a 5 - 8 micron section it seems
> that whatever tissue might have been present would be scraped away with
> the blade.
> Jerry Sedgewick
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